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. 2014 Jul 26;42(15):10161–10172. doi: 10.1093/nar/gku682

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Amino acid substitutions altering the Rcl1p–Bms1p interaction affect early steps of pre-rRNA processing in vivo. (A) Northern blot analysis of pre-rRNA levels in strains expressing altered versions of RCL1. Total RNAs were extracted from yeast strains harbouring a deletion of the chromosomal RCL1 open reading frame (rcl1::kan^R) and expressing plasmid-borne copies of wild-type or the indicated mutant versions of RCL1 (RCL1_C277R, RCL1_R327A, RCL1_C277R/R327A). The accumulation levels of the different pre-rRNA species were analysed by northern blot using specific probes (indicated on the right). As a control, RNAs extracted from wild-type cells (BY4741) were analysed in parallel. (B) PhosphorImager quantifications of the radioactive signals obtained in the northern blot experiments presented in panel A. The ratios of the 35S over 20S species intensities and of the 35S over 27SA₂ species intensities were calculated after background correction.