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. 2014 Jul 24;42(15):9949–9963. doi: 10.1093/nar/gku683

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Extension of control DNA and primer-templates that terminate in base mismatches. (A) Polymerisation of a control DNA lacking modified bases by Tgo-Pol and Tgo-Pol Z1. The primer-template shown in Figure 2A (X = T, Y = T; running start primer) was used. The main panel (A1) shows extension with all four dNTPs added. The smaller panel (A2) shows what takes place when only a single dNTP is used (with this primer-template combination, the first and second template bases are both dC; therefore, addition of two dGTPs represents ‘correct’ insertion). (B) Extension of a primer-template from a C:T base mismatch (Figure 2B) by Tgo-Pol and Tgo-Pol Z1. (C) Extension of a primer-template from a T:T base mismatch (Figure 2C) by Tgo-Pol and Tgo-Pol Z1.