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. 2014 Jul 24;42(15):9949–9963. doi: 10.1093/nar/gku683

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Extension of primer-templates that contain a single damaged base in the template. (A) Copying of a template containing an abasic site (Ab) (Figure 2A; X = T, Y = abasic site) by Tgo-Pol and Tgo-Pol Z1. (A1) shows the results found with the running start primer and all four dNTPs. (A2) uses the primer that initiates polymerisation from just before the abasic site (Figure 2A) and only a single dNTP to determine preferential incorporation opposite the lesion. (A3) utilises a primer that has a dA base across from the abasic site (Figure 2A), enabling extension from the dA:abasic site mis-pair to be evaluated. (B) Copying of a template containing a thymine glycol (Thgl) (Figure 2A; X = T, Y = thymine glycol) by Tgo-Pol and Tgo-Pol Z1. (B1), (B2) and (B3) are identical to A1, A2 and A3, except a thymine glycol replaces the abasic site.