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. 2014 Jul 24;42(15):9949–9963. doi: 10.1093/nar/gku683

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Extension of a primer-template containing a cis-syn thymine–thymine cyclobutane pyrimidine dimer (CPD) (Figure 2A; XY = dimer) by Tgo-Pol and Tgo-Pol Z1. (A1) Products produced using the running start primer in the presence of all four dNTPs. (A2) Products observed using a single dNTP and the primers that initiate synthesis immediately before the lesion (A2a) or opposite the first (3′) base of the lesion (A2b). (A3) Products found with the primer that commences polymerisation at the second (5′) base of the thymine dimer in the presence of all four dNTPs.