Table 2B. Individual changes introduced into the lacZα indicator gene of pSJ1 by Tgo-Pol Z1 (exo−).
Mutation type | Number | Frequency (%) | |||
---|---|---|---|---|---|
A→T/T→A | 8 | 7.4 | |||
A→C/T→G | 0 | 0 | |||
A→G/T→C | 2 | 1.9 | |||
G→A/C→T | 37 | 34.3 | |||
G→C/C→G | 37 | 34.3 | |||
G→T/C→A | 12 | 11.1 | |||
Insertions | 5 | 4.6 | |||
Deletions | 7 | 6.5 | |||
Total | 108 | 100 | |||
A→N/T→N | 10 | 10.4 | |||
G→N/C→N | 86 | 89.6 | |||
Transitions | 39 | 40.6 | |||
Transversions | 57 | 59.4 |
A total of 108 white colonies were completely sequenced in order to reveal the actual change in the DNA responsible for the white phenotype.