Figure 3.

Kinetics analysis of PRMT-mediated methylation of hnRNPK and its 4RK mutants. (a) Detection and quantification of PRMT1-mediated methylation of hnRNPK and diverse 4RK mutants over 120 min. HnRNPKs were incubated with GST-PRMT1 in the presence of [³H] S-adenosylmethionine (SAM), followed by SDS-PAGE. Proteins were stained with Coomassie blue (bottom). Methylation was detected through fluorography (top) and quantified using a liquid scintillation counter. (b) The methylation levels of all mutants relative to WT hnRNPK at the indicated times were determined and are shown as a stacked line chart.