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. 2014 Aug 11;42(15):9937–9948. doi: 10.1093/nar/gku728

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — The N-terminal part of VDAC34 is essential for an efficient interaction with tRNA. Two kinds of mutants were designed: (A) deleted versions of VDAC34 (D1–D7) and (B) chimeric proteins (C1–C9) in which segments of VDAC34 were replaced by segments of VDAC36. Numbers indicate position within VDAC sequences. The variants overexpressed in Escherichia coli were purified and used to perform NW experiments in the presence of Arabidopsis thaliana cytosolic tRNA^Ala in order to quantify percentage of interaction between mutant proteins and tRNA. Obtained values are represented on a diagram and are given in a summary table. They are the average of 2–9 independent experiments and correspond to the percentage of interaction of the mutant protein with tRNA compared to the wild-type VDAC34 interaction. Standard error is indicated for each value. Examples of NW experiments allowing the quantification of interactions are given as supplemental information (Supplemental Figure S4).