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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Biosens Bioelectron. 2014 Jul 11;0:117–123. doi: 10.1016/j.bios.2014.07.011

Figure 2.

Figure 2

Schematic of ELISA assay to determine efficiency of capturing PSA, detection antibody, and streptavidin. Row 1, ELISA assay of sample with known concentration of biotin-labeled PSA. Row 2, ELISA assay of supernatant from row 1 assay. Reduction in ELISA signal indicates how much biotin-labeled PSA is captured by anti-PSA beads. Row 4, ELISA assay of unlabeled PSA at same concentration as PSA in row 1, followed by biotin-labeled detection antibody. Comparing ELISA signal in row 4 vs. row 1 indicates how efficiently biotin-detection antibody is captured by bound PSA.