. Author manuscript; available in PMC: 2016 Jan 15.

Published in final edited form as: Biosens Bioelectron. 2014 Jul 11;0:117–123. doi: 10.1016/j.bios.2014.07.011

Table 2.

Enzyme-linked immunoassay to monitor efficiency of capture of analyte, detection antibody and streptavidin-peroxidase (SA-perox.) per Figure 2. Assay volumes were 44 μl.

sample	# anti-PSA beads	target molec.	# biot.-anti-PSA molec. added	# SA-perox. molec. added	OD₄₅₂
1	2*10⁵	2*10⁸ bio-PSA	none	4*10⁹	0.170
2	2*10⁵	supernatant of #1 after 1.5h	none	4*10⁹	0.044
3	2*10⁵	none	none	4*10⁹	0.052
4	2*10⁵	2*10⁸ PSA	2*10¹⁰	4*10⁹	0.181
OD₄₅₂ as function of number of SA-peroxidase molecules in solution
5				2*10⁸	0.764
6				2*10⁷	0.189
7				2*10⁶	0.036