Table 1.
Pin1 target regulation in cardiac myocytes and cardiac progenitor cells (CPCs)
| Pin1 Manipulation | Cell type | Target | Target Regulation | Cellular Outcome | Ref. |
|---|---|---|---|---|---|
| Knockdown (siRNA) | Cardiac myocytes | Akt | Decreased phosphorylation; Inhibition of downstream signaling | Decreased hypertrophy | (1) |
| Knockdown (siRNA); Overexpression (Adenovirus) | Cardiac myocytes | MEK | Decreased phosphorylation; Inhibition of downstream signaling | Decreased hypertrophy | (1) |
| Overexpression (Adenovirus) | Cardiac myocytes | Raf-1 | Increased phosphorylation (S259); Inhibition of downstream signaling | Decreased hypertrophy | (1) |
| Overexpression (Adenovirus) | Cardiac myocytes | Pin1 | Increased phosphorylation (S16) Inhibition of binding and function |
Increased hypertrophy | (25) |
| Knockdown (siRNA) | CPCs | Cyclin D | Decreased protein expression | Cell cycle arrest (G1/S) | (4) |
| Knockdown (siRNA); Overexpression (lentivirus) | CPCs | Cyclin B | Decreased protein expression | Cell cycle arrest (G1/S) Cell cycle arrest (G2/M) |
(4) |
| Knockdown (siRNA) Overexpression (lentivirus) |
CPCs | p53 | Increased protein expression Decreased protein expression |
Senescence Inhibits senescence |
(4) |
| Knockdown (siRNA) Overexpression (lentivirus) |
CPCs | Rb | Increased protein expression Decreased protein expression |
Senescence Inhibits senescence |
(4) |
| Knockdown (siRNA) Overexpression (lentivirus) |
CPCs | α-Smooth muscle actin | Decreased protein expression Increased protein expression |
Decreased differentiation Increased differentiation |
(4) |
| Knockdown (siRNA) Overexpression (lentivirus) |
CPCs | Cardiac Troponin T | Decreased mRNA expression Increased mRNA expression |
Decreased differentiation Increased differentiation |
(4) |
| Knockdown (siRNA) Overexpression (lentivirus) |
CPCs | c-kit | Decreased protein expression | Decreased stemness | (4) |
| Knockdown (siRNA) | CPCs | c-Myc | Decreased protein expression | Decreased proliferation, multipotency | (4) |
| Knockdown (siRNA) | CPCs | Oct4 | Decreased protein expression | Decreased multipotency | (4) |
| Knockdown (siRNA) Overexpression (lentivirus) |
CPCs | KLF4 | Decreased protein expression Increased protein expression |
Decreased multipotency Differential regulation of multipotency |
(4) |
| Knockdown (siRNA) Overexpression (lentivirus) |
CPCs | Nanog | Decreased protein expression Increased protein expression |
Decreased multipotency Differential regulation of multipotency |
(4) |