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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Mol Microbiol. 2014 Jul 30;93(5):957–974. doi: 10.1111/mmi.12711

Figure 2. Secretion of FedB-FlaA and CiaI-FlaA chimeric proteins from C. jejuni.

Figure 2

Immunoblot analysis of WT and chimeric proteins in whole-cell lysates (WCL) of C. jejuni strains or culture supernatants. WT C. jejuni and C. jejuni ΔflaA ΔflaB expressing WT FlaA, FlaAΔN′36, FedBN′14- FlaAΔN′36, FedBN′26- FlaAΔN′36 and CiaIN′26-FlaAΔN′36 from the flaA promoter in cis at the rdxA locus were grown in MH broth for 4 h at 37 °C in microaerobic conditions. The parental WT C. jejuni 81-176 SmR strain produces both the FlaA and FlaB flagellins. WCL and supernatant proteins were recovered and analyzed by immunoblotting with antisera specific for flagellin or RpoA, which served as a control for a cytoplasmic protein. Molecular weight markers are indicated in kDa.