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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Mol Microbiol. 2014 Jul 30;93(5):957–974. doi: 10.1111/mmi.12711

Figure 3. Analysis of secretion of flagellar proteins, FedB, CiaI, and FspA1 in C. jejuni mutants lacking putative flagellar chaperones.

Figure 3

Immunoblot analysis of proteins in (A) whole-cell lysates of C. jejuni or in culture supernatants. WT and C. jejuni isogenic mutants lacking putative flagellar chaperones or CiaB, which has been previously reported to be required for secretion of Cia proteins, were grown in MH broth for 4 h at 37 °C in microaerobic conditions. Whole-cell lysates (WCL) and supernatant proteins were recovered and analyzed by immunoblotting with antisera specific for each protein or RpoA, which served as a control for a cytoplasmic protein. The secreted flagellar proteins include the FlgD flagellar hook cap, the FlaA major flagellin, and the FlaB minor flagellin. The antisera for flagellins recognizes both FlaA and FlaB, which are of similar size. Molecular weight markers are indicated in kDa. (B) Electron micrographs of WT and isogenic C. jejuni 81-176 mutants lacking genes encoding putative flagellar chaperones. The bar represents 0.5 μm.