Table 1.
Strain | Invasion of T84 cells (% inoculum)a |
---|---|
Wild-type | 14.51 ± 1.43 |
ΔciaI | 7.51 ± 0.74* |
ΔciaI rdxA::ciaI | 12.89 ± 1.56 |
ΔciaI rdxA::ciaI Δ1 | 7.12 ± 1.07* |
ΔciaI rdxA::ciaI Δ4GH | 11.14 ± 1.01 |
Percent invasion was determined by comparing the number of intracellular bacteria surviving a 2 h gentamicin treatment of infected T84 cells compared to the number of bacteria in the infecting inoculum (approximately 3.0 × 106 cfu). Each assay was performed in triplicate, and at least three biological replicates were performed. The average percent invasion +/− standard error for each strain is presented. Statistically-significant differences in invasion between wild-type C. jejuni and mutant strains are indicated (* P-value < 0.05).