Table 1.

Cell-laden bilayered hydrogel design for the four formulations evaluated in this study. For each group, 6 animals were used.

Experimental Groups			MSC/MSC (12 implants)	MSC/OS (12 implants)	CG7/OS (12 implants)	CG14/OS (12 implants)
	OPF Hydrogel Precursor Solution
Cartilage Layer (Top 1 mm)	10 mM GMPs	mg per 100 mg OPF	22	22	22	22
		Cell Population	MSC	MSC	CG7	CG14
	Cell Suspension	CG Pre-differentiation Duration (Before Encapsulation)	0 Days	0 Days	7 Days	14 Days
		Encapsulation Density (million per mL precursor)	10	10	10	10
Subchondral Layer (Bottom 2 mm)	10 mM GMPs	mg per 100 mg OPF	22	22	22	22
		Cell Population	MSC	OS	OS	OS
	Cell Suspension	OS Pre-differentiation Duration (Before Encapsulation)	0 Days	6 Days	6 Days	6 Days
		Encapsulation Density (million per mL precursor)	10	10	10	10
Description