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. Author manuscript; available in PMC: 2014 Sep 2.
Published in final edited form as: Gene Ther. 2011 Jun 30;19(3):338–346. doi: 10.1038/gt.2011.96

Figure 5. Enhanced plasmid intracellular movement is prevented with siRNA against CREB.

Figure 5

(a) CREB knockdown via RNA interference. Western blot images show CREB protein levels are reduced around 75% in cell lysates after 48 hours incubation with siRNA against CREB (Ambion, Austin, TX) compared to cells transfected with a negative control scramble RNA. (b) Average cytoplasmic velocity of individual microinjected plasmids in scrambled RNA-transfected cells. Movement of individual DNA particles were tracked for 10 minutes and velocities analyzed as in Figure 4. (c) Average cytoplasmic velocity of individual microinjected plasmids in CREB siRNA-transfected cells. Plasmids were imaged and velocities recorded as in b. (d) Scatter plot showing the range of the average velocities for each of the four constructs. Bars represent median velocity for each construct. *, p<0.001. KD, CREB knockdown cells; SC, scrambled control.