Fig. 1.
Effect of laminin on cell adhesion, MMP-9 and TIMP-1 expression: a Graphical representation of cell adhesion assay. b MCF-7 cells were grown in SFCM in absence (lane 1) and presence of 10 μg/ml (lane 2) and 20 μg/ml (lane 3) LM for overnight (O.N.). Culture supernatants were subjected to gelatin zymography. Culture supernatant of HT-1080 cells grown in SFCM for 24 h was used as MMP-9 (92 kDa)/MMP-2 (72 kDa) marker (lane M). c MCF-7 cells were grown in absence (0) and in presence of 20 μg/ml LM coated dish and total RNA of each set was subjected to real time RT-PCR. In the given graph the CT value is inversely proportional to the mRNA expression of the samples. d Untreated and LM-treated cells were extracted and subjected to western blot using primary antibodies against TIMP-1. β-tubulin was used as internal control