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Comparative immunoblot: MCF-7 cells (300,000 cells/ml) were cultured in SFCM in absence (0) and presence of 20 μg/ml LM for overnight (O.N.). Cells were collected, extracted and equal protein (100 μg/lane) was subjected to western blot for FAK, pFAK (a) and PI3K, pPI3K, ERK, pERK (b) expression. β-tubulin was used as internal control
