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KLF4 is a downstream target of NGF and PC. PC12 Cells were treated with NGF or PC for indicated times (n = 3), then mRNA or protein level of KLF4 was measured by qPCR (a) or Western blotting (b), respectively. GAPDH was used as loading control. Both NGF and PC induced an increase in KLF4 mRNA and protein levels. Densitometric quantification KLF4 protein versus GAPDH was shown in c (****p < 0.0001 compared to 0 h)
