Fig. 3.

Increased FoxO6 phosphorylation and activation during aging and their inhibition by CR. a Nuclear extracts were prepared from young and old rat kidneys. Immunoprecipitation showed FoxO6 was physically associated with p-FoxO6 and 14-3-3β. b Western blot analyses for renal nuclear p-FoxO6 and FoxO6 were performed on nuclear proteins from AL and CR rats. The results shown are representative of three experiments. Results of one-factor ANOVA: ^** p < 0.01 vs. young age AL rats; ^*** p < 0.001 vs. old age AL rats