Fig. 4.

Effects of age and CR on gene expressions of MnSOD, catalase, and FoxO6-dependent genes. Effects of age and CR on the expression of catalase and MnSOD. Western blotting was used to determine catalase and MnSOD protein levels in the kidney tissues of young (6-month-old) and old (24-month-old) AL and CR rats. The results shown are representative of three experiments. Results of one-factor ANOVA: ^*** p < 0.001 vs. Y-AL control; ^*** p < 0.001 vs. Y-CR control; ^** p < 0.01 vs. age-matched AL rats; ^* p < 0.05 vs. age-matched CR rats. Y young, O old, AL ad libitum, CR calorie restriction