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. 2014 Aug 13;16(2):201–214. doi: 10.1016/j.chom.2014.07.005

Figure 2.

Figure 2

Expression of UL135

(A) HFFF-hCAR were infected with RAd-UL135 or an empty control vector (RAd-Ctrl) for 48 hr, and HFFF were mock infected (M) or infected with HCMV strain Merlin or MerlinΔUL135 for the indicated times. Proteins were separated by SDS-PAGE, and western blot was performed for UL135 (V5 antibody) or actin.

(B) HFFF-hCAR were infected with RAd-UL135 or RAd-Ctrl for 48 hr, and samples were stained for UL135 (V5 antibody) and giantin (golgi marker). HFFF were infected with the indicated strains of HCMV for 48 hr and stained for UL135 (V5 antibody) or IE1/IE2 (MerlinΔUL135) and giantin.

(C) HFFF were infected with the indicated strains of HCMV and stained for either surface or intracellular UL135 expression 48 hr later.

(D and E) Brightfield images of HFFF-hCAR infected with RAd-UL135 or RAd-Ctrl (D) and HFFF (E) mock infected or infected with HCMV strain Merlin or MerlinΔUL135 for 48 hr.