Figure 4.

UL135 Interacts with the WAVE2 Complex and Talin

(A) HFFF-hCAR were infected with RAd-UL135 or RAd-Ctrl, and HFFF were infected with HCMV strain Merlin or MerlinΔUL135. Samples were lysed 48 hr postinfection, and immunoprecipitation was performed for the V5 tag on UL135. Proteins were separated by SDS-PAGE, and western blot was performed for the indicated proteins.

(B–I) HFFF-hCAR were infected with RAd-UL135 or RAd-Ctrl (B, D, F, and H), and HFFF were infected with HCMV strain Merlin or MerlinΔUL135 or mock infected (C, E, G, and I). Samples were fixed and stained for UL135 (V5 antibody) and ABI1 (B and C), WAVE2 (D and E), or talin (H and I) 48 hr postinfection. Samples were lysed, and SDS-PAGE was performed 48 hr postinfection (F) or at the indicated time points (G) followed by western blot for the indicated proteins.