Table 3.
Genes differentially regulated upon combination therapy in comparison to vaccinated mice
| Pathway/function | Downregulated genes |
|---|---|
| Genes associated with tumor invasiveness | Adam18 (6.7), Adam9 (1.6), Tgfbi (1.7), Mmp12 (1.8), Mmp3 (2.0), Mmp10 (2.2), Slc7a2 (2.4) |
| Tumor associated factors | Ly6i (3.1), Lrrc15 (2.4), Mamdc2 (2.4), Tm2d2 (2.1), Gpr97 (2.3), Grem1 (2.5), Dok4 (2.4), Tacc1 (2.3), Inhba (2.0), Ifitm1 (2.4) |
| Pathway/function | Upregulated genes |
| Genes associated with tumor suppresion | Phlda1 (2.3), Cxcl14 (2.4), Cd209a (2.4), Timp1 (1.9), Sfrp4 (3.9), Sulf1 (1.8), Nr1d1 (1.8), Ogn (5.0), Scara3 (2.3), Dpt (5.0) |
C57BL/6 mice (4 mice per group) were challenged subcutaneously on the right limb with 3 × 105 syngenic E.G7-OVA tumor cells. Mice were treated with the radioimmunotherapy starting at day 13 (local radiation of 6 Gy applied in 3 equal doses on 3 consecutive days and three vaccination with OVA-encoded mRNA – 32 μg, 2x week). OVA-encoded mRNA vaccinated mice served as controls. After 7 days tumors were exercised, RNA was isolated and gene expression was analyzed. Differentially expressed genes were identified by statistical analysis with the p ≤ 0.05 and a fold change of at least 1.5. The exact fold change is presented in the brackets.