Table 2.
Lists of genes significantly altered by exposure to pristine CeO 2 (Part A), NB-DA (Part B) and NB-DL (Part C), and belonging to the canonical pathway “mitochondrial dysfunction”
| Part A: genes altered by pristine CeO2 (n = 27) | ||||
|---|---|---|---|---|
| Symbol | Entrez Gene Name | Fold Change | p-value | ID Agilent |
| ATP5J | ATP synthase, H + transporting, mitochondrial F0 complex, subunit F6 | −1,935 | 8,67E-03 | A_23_P154832 |
| COX4I1 | cytochrome c oxidase subunit IV isoform 1 | −2,313 | 4,97E-03 | A_23_P141029 |
| COX5B | cytochrome c oxidase subunit Vb | −2,312 | 4,98E-03 | A_23_P51069 |
| COX6A1 | cytochrome c oxidase subunit VIa polypeptide 1 | −2,312 | 4,99E-03 | A_32_P168247 |
| COX6B1 | cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) | −2,306 | 4,87E-03 | A_23_P108244 |
| COX6C | cytochrome c oxidase subunit VIc | −2,088 | 3,08E-03 | A_23_P8900 |
| COX7A2 | cytochrome c oxidase subunit VIIa polypeptide 2 (liver) | −2,311 | 4,99E-03 | A_23_P81690 |
| COX7C | cytochrome c oxidase subunit VIIc | −2,312 | 4,99E-03 | A_23_P110811 |
| COX8A | cytochrome c oxidase subunit 8A (ubiquitous) | −2,312 | 5,00E-03 | A_23_P52639 |
| CYB5R3 | cytochrome b5 reductase 3 | −2,312 | 4,99E-03 | A_23_P502224 |
| NDUFA3 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9 kDa | −2,052 | 4,57E-03 | A_23_P208540 |
| NDUFA4 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9 kDa | −2,312 | 4,98E-03 | A_23_P145777 |
| NDUFA6 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14 kDa | −2,037 | 6,10E-03 | A_23_P91769 |
| NDUFA8 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19 kDa | −2,200 | 4,97E-03 | A_23_P43566 |
| NDUFB2 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8 kDa | −2,175 | 2,93E-03 | A_24_P406301 |
| NDUFB4 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15 kDa | −2,301 | 4,77E-03 | A_23_P69468 |
| NDUFB8 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19 kDa | −1,963 | 4,31E-03 | A_24_P346886 |
| NDUFB9 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22 kDa | −2,311 | 4,99E-03 | A_23_P157669 |
| NDUFS2 | NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49 kDa (NADH-coenzyme Q reductase) | −1,561 | 5,68E-04 | A_23_P149470 |
| NDUFS5 | NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15 kDa (NADH-coenzyme Q reductase) | −2,311 | 4,98E-03 | A_23_P10463 |
| NDUFS7 | NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20 kDa (NADH-coenzyme Q reductase) | −2,079 | 3,89E-03 | A_23_P165086 |
| NDUFS8 | NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23 kDa (NADH-coenzyme Q reductase) | −1,904 | 7,63E-03 | A_23_P86774 |
| NDUFV1 | NADH dehydrogenase (ubiquinone) flavoprotein 1, 51 kDa | −1,704 | 1,08E-02 | A_23_P127353 |
| OGDH | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) | −1,513 | 3,02E-03 | A_23_P123133 |
| PRDX5 | peroxiredoxin 5 | −1,946 | 5,32E-03 | A_24_P155378 |
| UQCRFS1 | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 | −2,031 | 3,93E-03 | A_23_P153586 |
| UQCRH | ubiquinol-cytochrome c reductase hinge protein | −2,312 | 4,99E-03 | A_32_P54137 |
| Part B: genes altered by NB-DA (n = 10) | ||||
| Symbol | Entrez Gene Name | Fold Change | p-value | ID Agilent |
| ATP5B | ATP synthase, H + transporting, mitochondrial F1 complex, beta polypeptide | −1,575 | 2,48E-02 | A_23_P33216 |
| ATP5C1 | ATP synthase, H + transporting, mitochondrial F1 complex, gamma polypeptide 1 | −1,525 | 3,84E-02 | A_23_P63655 |
| COX6A2 | cytochrome c oxidase subunit VIa polypeptide 2 | −2,028 | 4,43E-02 | A_23_P401524 |
| COX6B2 | cytochrome c oxidase subunit VIb polypeptide 2 (testis) | −1,522 | 2,45E-02 | A_23_P78571 |
| CYCS | cytochrome c, somatic | −1,674 | 3,09E-02 | A_24_P376556 |
| NDUFA5 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13 kDa | −1,815 | 2,45E-02 | A_32_P96641 |
| PRDX3 | peroxiredoxin 3 | −2,65 | 2,51E-02 | A_23_P63751 |
| PSEN2 | presenilin 2 (Alzheimer disease 4) | −1,647 | 2,98E-02 | A_23_P96985 |
| SDHB | succinate dehydrogenase complex, subunit B, iron sulfur (Ip) | −1,646 | 2,46E-02 | A_23_P149649 |
| SDHD | succinate dehydrogenase complex, subunit D, integral membrane protein | −1,841 | 2,39E-02 | A_23_P138967 |
| Part C: genes altered by NB-DL (n = 3) | ||||
| Symbol | Entrez Gene Name | Fold Change | p-value | ID Agilent |
| COX11 | COX11 cytochrome c oxidase assembly homolog (yeast) | −1,723 | 3,28E-02 | A_24_P404204 |
| SDHC | succinate dehydrogenase complex, subunit C, integral membrane protein, 15 kDa | −2,264 | 3,53E-02 | A_24_P233850 |
| SDHD | succinate dehydrogenase complex, subunit D, integral membrane protein | −2,03 | 4,62E-02 | A_32_P60185 |
Although the same pathway is activated, there are, stricto sensu, no common genes altered by pristine CeO2 and NB-DA or NB-DL in the mitochondrial dysfunction pathway. This demonstrates the interest of a pathway-driven analysis compared to the usual gene-driven analysis.