Figure 6.

FAS mRNA colocalizes with Wig-1 protein in SGs. RNA FISH staining for FAS mRNA together with anti-Wig-1 (a), anti-TIAR (b) or anti-HEDLS (c) antibody in untreated or treated (0.5 mM arsenite) U2OS cells. TIAR and HEDLS proteins were used as SG and processing body marker, respectively. Arrows indicate the positions of overlapping FAS mRNA and Wig-1 or TIAR proteins. Scale bars, 20 μm. Blue dye shows DAPI DNA staining. Representative images from one of two independent experiments are shown. (d) Model for the role of Wig-1 in regulation of cell death and survival in the p53 stress response. (e) Model for Wig-1-mediated post-transcriptional regulation of FAS mRNA.