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. 2014 Jun 27;30(10):2663–2671. doi: 10.1007/s11274-014-1690-4

Table 2.

Primers used in the m-PCR reaction

Pathogen Primer Sequence(5′-3′) Size (bp) Conc. (µM) Reference
E. coli mdh (F) GGT ATG GAT CGT TCC GAC CT 304 0.1 Tarr et al. (2002)
mdh (R) GGC AGA ATG GTA ACA CCA GAG T
EIEC ial (F) GGT ATG ATG ATG ATG AGT CCA 650 0.2 López-Saucedo et al. (2003)
ial (R) GGA GGC CAA CAA TTA TTT CC
EHEC/Atypical EPEC eaeA (F) CTG AAC GGC GAT TAC GCG AA 917 0.3 Aranda et al. (2004)
eaeA (R) CCA GAC GAT ACG ATC CAG
Typical EPEC bfpA (F) AAT GGT GCT TGC GCT TGC TGC 410 0.3 Aranda et al. (2004)
bfpM (R) TAT TAA CAC CGT AGC CTT TCG CTG AAG TAC CT From this study
EAEC eagg (F) AGA CTC TGG CGA AAG ACT GTA TC 194 0.2 Pass et al. (2000)
eagg (R) ATG GCT GTC TGT AAT AGA TGA GAA C
EHEC stx1 (F) ACA CTG GAT GAT CTC AGT GG 614 0.5 Moses et al. (2006)
stx1 (R) CTG AAT CCC CCT CCA TTA TG
stx2 (F) CCA TGA CAA CGG ACA GCA GTT 779 0.3 Moses et al. (2006)
stx2 (R) CCT GTC AAC TGA GCA CTT TG
ETEC lt (F) GGC GAC AGA TTA TAC CGT GC 360 0.1 Pass et al. (2000)
lt (R) CGG TCT CTA TAT TCC CTG TT
st (F) TTT CCC CTC TTT TAG TCA GTC AAC TG 160 0.5 Pass et al. (2000)
st (R) GGC AGG ATT ACA ACA AAG TTC ACA
E. coli toxin astA (F) GCC ATC AAC ACA GTA TAT CC 106 0.3 Kimata et al. (2005)
astA (R) GAG TGA CGG CTT TGT AGT C
External control gapdh (F) GAG TCA ACG GAT TTG GTC GT 238 0.3 Mbene et al. (2009)
gapdh (R) TTG ATT TTG GAG GGA TCT CG

NB: F forward primer, R reverse primer