Table 2.
Pathogen | Primer | Sequence(5′-3′) | Size (bp) | Conc. (µM) | Reference |
---|---|---|---|---|---|
E. coli | mdh (F) | GGT ATG GAT CGT TCC GAC CT | 304 | 0.1 | Tarr et al. (2002) |
mdh (R) | GGC AGA ATG GTA ACA CCA GAG T | ||||
EIEC | ial (F) | GGT ATG ATG ATG ATG AGT CCA | 650 | 0.2 | López-Saucedo et al. (2003) |
ial (R) | GGA GGC CAA CAA TTA TTT CC | ||||
EHEC/Atypical EPEC | eaeA (F) | CTG AAC GGC GAT TAC GCG AA | 917 | 0.3 | Aranda et al. (2004) |
eaeA (R) | CCA GAC GAT ACG ATC CAG | ||||
Typical EPEC | bfpA (F) | AAT GGT GCT TGC GCT TGC TGC | 410 | 0.3 | Aranda et al. (2004) |
bfpM (R) | TAT TAA CAC CGT AGC CTT TCG CTG AAG TAC CT | From this study | |||
EAEC | eagg (F) | AGA CTC TGG CGA AAG ACT GTA TC | 194 | 0.2 | Pass et al. (2000) |
eagg (R) | ATG GCT GTC TGT AAT AGA TGA GAA C | ||||
EHEC | stx1 (F) | ACA CTG GAT GAT CTC AGT GG | 614 | 0.5 | Moses et al. (2006) |
stx1 (R) | CTG AAT CCC CCT CCA TTA TG | ||||
stx2 (F) | CCA TGA CAA CGG ACA GCA GTT | 779 | 0.3 | Moses et al. (2006) | |
stx2 (R) | CCT GTC AAC TGA GCA CTT TG | ||||
ETEC | lt (F) | GGC GAC AGA TTA TAC CGT GC | 360 | 0.1 | Pass et al. (2000) |
lt (R) | CGG TCT CTA TAT TCC CTG TT | ||||
st (F) | TTT CCC CTC TTT TAG TCA GTC AAC TG | 160 | 0.5 | Pass et al. (2000) | |
st (R) | GGC AGG ATT ACA ACA AAG TTC ACA | ||||
E. coli toxin | astA (F) | GCC ATC AAC ACA GTA TAT CC | 106 | 0.3 | Kimata et al. (2005) |
astA (R) | GAG TGA CGG CTT TGT AGT C | ||||
External control | gapdh (F) | GAG TCA ACG GAT TTG GTC GT | 238 | 0.3 | Mbene et al. (2009) |
gapdh (R) | TTG ATT TTG GAG GGA TCT CG |
NB: F forward primer, R reverse primer