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Blocking test of Hm2E8b antibody to 2E8-FITC. Binding activity of parental 2E8 antibody was partially blocked by chimeric antibody Hm2E8b. The panel includes four groups from left to right, representing background control (GAH-Fc-FITC), positive control (2E8-FITC), Hm2E8b+2E8-FITC, incubated with Hm2E8b twice+2E8-FITC. Two parameters as percentage of positive cells (%Gate) and mean fluorescence intensity (Geo mean) were used to assess the binding ability of the Hm2E8b antibody.
