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Competitive inhibition assay of Hm2E8b antibody. Decrease of 2E8-FITC binding with increasing concentrations of inhibitors Hm2E8b and 2E8 was monitored by flow cytometry. Approximately 4.5-times greater concentrations of Hm2E8b than that of the parental 2E8 antibody were needed to achieve half-maximum inhibition.
