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. 2014 Aug 1;33(4):261–269. doi: 10.1089/mab.2013.0085

FIG. 3.

FIG. 3.

1B9B9 suppressed the activity of ERK1,2 and Akt in HUVECs. (A) (upper) Serum-starved HUVECs were stimulated for 30 min with FGF-2, which had been incubated with 1B9B9 (1 μg/mL, 5 μg/mL, 10 μg/mL) or IgG (10 μg/mL) for 1 h. Thereafter lysates were immunoblotted with pAkt or pERK1,2 antibodies. (lower) Densitometric quantitation of each protein using β-actin for normalization. *p<0.05 vs. FGF-2+IgG, by one-way ANOVA; values are means±SE (n=5). (B) HUVECs were incubated with IgG (10 μg/mL) or 1B9B9 (1 μg/mL, 5 μg/mL, 10 μg/mL) for 1 h under serum- and growth factor-starved conditions. (upper) Phosphorylation of Akt or ERK1,2 was analyzed by immunoblotting. (lower) Densitometric quantitation of each protein using β-actin for normalization. *p<0.05 vs. IgG by one-way ANOVA; values are means±SE (n=4).