FIG. 6.

Comparison of 4-HNE-histidine adduct levels (A) and nitrotyrosine adduct levels (B) in the neural retina of rats exposed to light after 2-week dark adaptation. HNE-histidine adduct levels, as determined by ELISA, in rats treated with or without the multifunctional antioxidant JHX-4 and Optixcare EH suggests that HNE levels were reduced by treatment. Similar conclusions were obtained with ELISA measurements of nitrotyrosine-modified proteins in rats treated with or without these antioxidants. The JHX-4 data have been previously published.³⁵ Mean±standard deviation (SD); n=6. *P<0.05 compared with the untreated, nonlight-damaged (NLD) group. #P<0.05 compared with the untreated light damaged (LD) group. ELISA, enzyme-linked immunosorbent assay.