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. 2014 Aug 15;15:17. doi: 10.1186/1471-2199-15-17

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Copyright © 2014 Agustina et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Interaction between hMYH and hRad9. (A) HEK293 cells were transfected with c-myc and FLAG-tagged hRad9, or c-myc-tagged hMYH and FLAG-tagged-hRad9 as indicated. Co-IP was performed with anti-c-myc antibody, and the immunoprecipitates were immunoblotted with anti-FLAG and anti-c-myc antibodies. (B) Lysates from untransfected HEK293 cells were used for the immunoprecipitation of hMYH with an anti-hMYH antibody. Immunoblotting was performed using anti-hMYH and anti-hRad9 antibodies. Lane 1: input sample, lanes 2 and 3: proteins immunoprecipitated with mouse IgG or anti-hMYH antibody. (C) HEK293 cells were transfected with siHus1 or siGFP. Total cell lysates were used for co-IP with hRad9 antibody. (D) Pull-down of purified His-hRad9 with GST-hMYH bound to glutathione-Sepharose beads. Lane 1: purified His-hRad9, lanes 2 and 3: pull-downs with GST and GST-hMYH, respectively.