Figure 4. Dsh::GFP vesicles move preferentially toward the distal side of the cell.

(A and B) Timelapse images of Dsh::GFP vesicles in 24 hAPF pupal wing. The majority of Dsh::GFP vesicles displayed directed, fast transcytotic movement with a distal bias (A). A minority of vesicles moved slowly along irregular paths (B) (derived from Video 2 and Video 3). (A′ and B′) Overlays of 73 frames from timelapse videos in A and B. Proximal is to the left and anterior is at the top. (C) The ratios of transcytosing Dsh::GFP vesicles at different pupal ages (n ≥ 1192). (D) Ratio of Dsh::GFP vesicles moving toward proximal, distal, anterior or posterior, or not moving (n = 50). (E) Similar plot of Vang::YFP vesicles showing absence of directed trafficking (n = 81). (F) Dsh::GFP vesicle movement inside ft clones showing little net movement and no significant bias (n = 42). (G) Dsh¹::GFP vesicles in dsh¹ wings showing a bias to P-D vesicle movement among vesicles showing net movement (n = 120). Numbers were too small to test significance of a possible difference between P and D. Scale bars, 5 μm. Significant differences between proximal and distal at p ≤ 0.05 using the binomial test are marked with *.

DOI: http://dx.doi.org/10.7554/eLife.02893.017

Figure 4—figure supplement 1. Vesicle identification and tubulin staining.

(A) 24 hAPF Dsh::GFP wing co-labeled for Sec5 and Rab5. Many Dsh::GFP vesicles do not label with Sec5 or Rab5 (pink arrowheads), while some stain for Sec5 (yellow arrowhead) or Rab5 (blue arrowhead). (B) Tubulin staining in fz and dsh¹ mutant flies. Compare to Figure 1—figure supplement 1C.

DOI: http://dx.doi.org/10.7554/eLife.02893.018