Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Monoamination of diketone 3a to yield Δ1-piperideine 6a. Reagents and conditions: lyophilised E. coli cells containing the overexpressed ω-TA (20 mg), diketone 3a (12 mg, 50 mm), PLP (1 mm), NAD+ (1 mm), ammonium formate (150 mm), D- or L-alanine (500 mm), AlaDH (12 U), FDH (11 U), organic cosolvent and KPi buffer (100 mm, pH 7.0), 30 °C, 24 h in an Eppendorf orbital shaker (700 rpm).
