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Conversions for the monoamination of diketone 3a in the presence of various organic cosolvents employing (R)- and (S)-selective ω-TAs. Reagents and conditions: diketone 3a (6 mg/mL, 25 mm), lyophilised E. coli cells containing overexpressed ω-TA (20 mg), PLP (1 mm), NAD+ (1 mm), ammonium formate (150 mm), D- or L-alanine (500 mm), AlaDH (12 U), FDH (11 U), organic cosolvent, K-phosphate buffer (100 mm, pH 7.0), 30 °C, 24 h in an Eppendorf orbital shaker (700 rpm).
