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. 2013 Apr 15;2013(16):3397–3402. doi: 10.1002/ejoc.201300157

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© 2013 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Reductive amination of diketone 3a in an aqueous two-phase system: (▪) (R)-Arthrobacter sp., (•) A. citreus. Reagents and conditions: diketone 3a (25 mm) dissolved in 5 vol.-% DMF, lyophilised E. coli cells containing the overexpressed ω-TA (20 mg), PLP (1 mm), NAD⁺ (1 mm), ammonium formate (150 mm), D- or L-alanine (500 mm), AlaDH (12 U), FDH (11 U), 5–40 vol.-% n-heptane, KPi buffer (100 mm, pH 7.0), 30 °C, 24 h, 700 rpm.