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. 2014 Sep 1;206(5):655–670. doi: 10.1083/jcb.201401070

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© 2014 Ashrafi et al.

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Figure 5. — Parkin is recruited to axonal mitochondria damaged with mt-KR. (A) YFP-Parkin accumulates on a fraction of axonal mitochondria in the outlined area of mt-KR activation. (B) Line scan of the axon in A with cyan arrowheads marking two YFP-Parkin–positive mitochondria. (C and D) YFP-Parkin remained diffuse despite irradiation in the outlined area when mt-DsRed replaced mt-KR. Mitochondria with YFP-Parkin levels more than twice the background were scored as Parkin-positive here and in subsequent figures. Orange and brown arrowheads denote corresponding points in images and line scans. (E) Frequency of YFP-Parkin recruitment to irradiated mitochondria. n = 131–140 mitochondria from 12 transfections. **, P < 0.001. Error bars represent means ± SEM. AU, arbitrary unit. Bars, 5 µm.