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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Curr Protoc Immunol. 2014 Aug 1;106:5.10.1–5.10.12. doi: 10.1002/0471142735.im0510s106

Figure 2. A schematic overview of the steps involved in labeling the probe peptide with a fluorochrome.

Figure 2

(A) Set up the labeling reaction mix and incubate at room temperature for 1 hour. (B) Representative elution profile of fluorochrome Alexa488-TFP (first panel, peak (a)), unlabeled FRR-HA306-318 (second panel, peak (b)), labeling reaction mix (third panel), and Alexa488-labeled FRR-HA306-318 (fourth panel, peak (c)). Peak (a) in the second panel comes from the residual fluorochrome Alexa488-TFP in the last run. The fifth panel shows the Buffer B gradient.