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. 2014 Aug 14;15(1):90. doi: 10.1186/s12931-014-0090-5

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© Nakada et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Frequencies of total IL-17 expressing cells and of IL-17-γδ cells are increased in OVA/CFA sensitized mice treated with a γδ TCR stimulatory antibody. OVA/CFA sensitized mice were IV injected with a γδ TCR stimulatory antibody (UC7-13D5) or isotype control before airway challenge. BAL fluid cells were stimulated with PMA/ionomycin and stained with α-γδ TCR and α-IL-17 antibodies. (A) Representative flow cytometry plots of BAL fluid cells. The mean frequencies (left panels) and numbers (right panels) of (B) IL-17-γδ and (C) IL-17⁺ BAL fluid cells are presented for 5 total mice per group from 2 independent experiments. (B-C) Unpaired, two-tailed t-test. *p < 0.05.