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. 2014 Aug 8;124(9):3863–3878. doi: 10.1172/JCI72189

Figure 8. The VEGF-C/VEGFR3 pathway regulates in vivo and in vitro MΦ plasticity and activation.

Figure 8

(A and B) Mice undergoing 2 cycles of DSS treatment were injected with Ad-hVEGF-C or anti-VEGFR3 Ab. Colonic expression of M2 (A) and M1 (B) MΦ-associated genes was examined by real-time PCR analysis for Ym1, Arg1, Fizz1, Cox2, and iNOS; by ELISA for IL-10; and by FACS for MHCII and CD80 after 24 hours in vitro. Data represent the means ± SEM per group (n = 4–6/group). (A and B) **P < 0.01 versus DSS alone. (C and D) BM-derived MΦs were cultured and stimulated in vitro with LPS (M1), IL-4 (M2), VEGF-C, and VEGF-C plus mF431C1 for 24 hours. Expression of the indicated genes was then examined by real-time PCR, ELISA, or FACS. Data represent the means ± SEM of 3 independent experiments. **P < 0.01 versus untreated (UN); §P < 0.001 versus VEGF-C alone.