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. 2014 Aug 1;124(9):3767–3780. doi: 10.1172/JCI66603

Figure 9. FOXA2 regulates AGR2 and MUC2.

Figure 9

(A) FOXA2 and AGR2 Western blot, following vector (V) or FOXA2 (F) electroporation in NES-B3T cells and electroporation (e) or transfection (t) in NES-B10T cells. OE33 cells served as a positive control. (B) FOXA2 and AGR2 Western blot, following FOXA2 knockdown by 25 nM scrambled or 3 separate FOXA2 siRNAs (si1–si3) in BAR-10T cells. (C) MUC2 immunofluorescence of NES-B3T cells electroporated with vector or FOXA2. BAR-10T cells and a Barrett’s tissue specimen served as positive controls. Scale bars: 50 μm. (D) FOXA2, AGR2, CDX2 qRT-PCR and MUC2 RT-PCR following electroporation of NES-B3T and NES-B10T cells with FOXA2 (Western blot). (E) AGR2 qRT-PCR and MUC2 RT-PCR following FOXA2 siRNA knockdown in BAR-T cells (Western blot).