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. 2014 Jul 25;124(9):3713–3724. doi: 10.1172/JCI64628

Figure 1. CBD prevents excessive lipogenesis induced by “pro-acne agents” in human SZ95 sebocytes.

Figure 1

(A, B, D, and E) Semiquantitative determination of lipid synthesis for (A) control, (B) 10 μM CBD, (D) 30 μM AEA, and (E) 30 μM AEA plus 10 μM CBD (sebum droplets: Oil Red O staining, red; nuclei: hematoxylin, blue). Scale bars: 10 μm. (C) Quantitative determination of lipid synthesis (Nile Red staining). **P < 0.01, ***P < 0.001 compared with the AEA-treated group. PL, polar lipids; NL, neutral lipids. (F) Neutral lipid synthesis (Nile Red staining). ***P < 0.001 compared with the respective AA- or LA-T–treated group. (C and F) Data are expressed as the percentage of the vehicle control (mean ± SEM of 4 independent determinations). The solid line indicates 100%. Two additional experiments yielded similar results. (G) Analysis of the sebaceous lipidome. Sebaceous lipid classes were analyzed by HPLC-ToF/MS in sebocytes. CH, free cholesterol; CE, cholesteryl esters; WE, wax esters; DG, diacylglycerols; TG, triacylglycerols; SQ, squalene; FFA, free fatty acids. Results are expressed as the percentage of the vehicle control (mean ± SD of 3 independent determinations). The solid line indicates 100%. Two additional experiments yielded similar results. *P < 0.05, **P < 0.01, ***P < 0.001.