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. 2014 Jul 25;124(9):3713–3724. doi: 10.1172/JCI64628

Figure 6. Anti-acne actions of CBD are mediated by parallel, partly independent signaling mechanisms.

Figure 6

(A) CyQUANT proliferation assay after 72-hour treatments. *P < 0.05 compared with the vehicle control. #P < 0.05. The solid line indicates the level of the 24-hour vehicle control. Dashed line indicates the level of the 72-hour vehicle control. Results are expressed as the percentage of the 24-hour vehicle control (mean ± SEM of 4 independent determinations). (B) TNFA mRNA expression following 24-hour LPS treatments with or without CBD and HC. *P < 0.05 compared with the vehicle control; #P < 0.05 compared with the CBD-free LPS-treated group. Data are presented using the ΔΔCT method; peptidyl-prolyl isomerase A–normalized (PPIA-normalized) TNFA mRNA expression of the vehicle control was set as 1. Data are expressed as mean ± SD of 3 independent determinations. Two additional experiments yielded similar results. (C) Validation of the key microarray results. mRNA expression of various target genes following 24-hour CBD treatments with or without HC. **P < 0.01, ***P < 0.001 compared with the vehicle control. ###P < 0.001. Data are presented using the ΔΔCT method; PPIA-normalized mRNA expression of the vehicle control was set as 1 (solid line). Data are expressed as mean ± SD of 3 to 6 independent determinations. Two additional experiments yielded similar results.