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. Author manuscript; available in PMC: 2014 Sep 2.
Published in final edited form as: Methods Mol Biol. 2010;649:437–455. doi: 10.1007/978-1-60761-753-2_27

Fig. 27.6.

Fig. 27.6

IP-western blot analysis of the zinc finger protein ZBTB16. ZBTB16 was immunoprecipitated from LNCaP nuclear extracts using 1, 2, and 4 μg of either control IgG or anti-ZBTB16 antibody. ZBTB16 protein was detected in whole cell extract (WCE) prior to immunoprecipitation and in immunoprecipitations using ZBTB16 antibody, but not IgG. Based on the success of the antibody in these standard immunoprecipitations, it is expected that the antibody would be appropriate for use in ChIP assays.