| 24 μL | DNA from Step 3.5.3 |
| 25 μL | 2× Phusion DNA polymerase |
| 0.5 μL | Illumina Genomic PCR primer 1.1 |
| 0.5 μL | Illumina Genomic PCR primer 2.1 |
| 50 μL | Total reaction volume |
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