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. 2014 Sep 2;5:462. doi: 10.3389/fmicb.2014.00462

FIGURE 1.

FIGURE 1

tmRNA and SmpB binding in the decoding center. (A) Crystal structure of the Thermus thermophilus tmRNA–SmpB complex bound to EFTu on the 70S ribosome, trapped by kirromycin in the pre-accommodation state. Only the tRNA-like domain of tmRNA is included. Rendered using the coordinates from PDB 4ABR and 4ABS (Neubauer et al., 2012). (B) SmpB engages 16S rRNA nucleotides A1492, A1493, and G530 in the decoding center. The C-terminal tail extends into the mRNA channel. (C) Conserved SmpB residues interact with G530 and nearby nucleotides. T. thermophilus Tyr126 corresponds to E. coli His136. (D) Logo of the C-terminal tail from 470 SmpB proteins (Andersen et al., 2006) with the E. coli sequence shown underneath.