Table 2.
Kinetic parameters of Rb+ uptake mediated by AtHAK5 and its mutant forms in yeast.
| Vmax (nmol mg-1 min-1) | Rb+ Km (μM) | K+ Ki (μM) | Na+ Ki(mM) | Cs+ Ki (μM) | |
|---|---|---|---|---|---|
| WT* | 0.5 ± 0.3 | 12.6 ± 0.3 | 12 | 10 | 16 |
| L776H* | 5.2 ± 0.3 | 8.1 ± 1.0 | 12 | 10 | 16 |
| Mut11 | 4.1 ± 0.2 | 2.5 ± 0.7 | 5.7 ± 0.1 | 12.3 ± 2.1 | 21.3 ± 0.8 |
| F130S | 1.0 ± 0.2 | 0.1 ± 0.1 | 0.3 ± 0.2 | 0.6 ± 0.3 | 0.3 ± 0.2 |
*
From (Rubio et al., 2000).
Values at the average of at least three replicates ± standard error. Vmax (nmol mg−1 min−1) and Km (μM) values for Rb+ uptake and Ki values for inhibition of Rb+ uptake by K+ (μM), Na+ (mM), and and Cs+ (μM).