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Estradiol (E2) increased the invasion of 4T1 cells. Murine 4T1 cells were incubated in biocoated invasion chambers for 24 h and invasiveness was evaluated by counting cells that travelled through the biocoated PET membrane per field. Data (mean ± SEM) are normalized to the untreated control cells (C) and are representative of two independent experiments. ** Indicates significant difference at the level of 0.01 when compared to the untreated cells
