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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Stem Cells. 2014 Apr;32(4):860–873. doi: 10.1002/stem.1584

Figure 2. Levels of Lin28a transcript and LIN28A protein in Lin28a mutant testis samples are significantly reduced in adult mice.

Figure 2

(A) RT-PCR analysis of Lin28a, Lin28b and iCre transcript in control and Lin28a mutant testis showing loss of Lin28a while Lin28b and iCre expression remains unaltered. (B) Whole mount immunostaining of isolated seminiferous tubules from control (top panels) and Lin28a mutant samples (bottom panels) stained with LIN28A and PLZF antibodies confirming the absence of LIN28A in spermatogonia of mutant mice. Boxed area shows PLZF+LIN28A− As cells. Scale bar = 20 um.