Fig. 1.
Activity of Sanguisorba officinalis extract (SOE) against HIV-1ADA, HIVHxB2, and HIVVSV. Serially diluted SOE was added to GHOST-CCR5 and GHOST-CXCR4 infected with (A) HIV-1ADA and (B) HIVHxB2, respectively. HIVVSV was tested as a negative control. The luciferase level was measured 2 days postinfection. All results are means ± standard errors of the means from three independent experiments. To test SOE cytotoxicity, (C) GHOST(3)-CD4-CCR5 and (D) GHOST(3)-CD4-CXCR4 cells were cultured in serially diluted SOE at 37°C for 48 hours in 5% CO2. Cell viability was then measured using the Promega CellTiter-Glo Luminescent Cell Viability Assay kit. The data represent the mean ± standard deviation of triplicate experiments.