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(A) Rates of O2 reduction to form either H2O (blue) or ROS (red) catalyzed by 6 μM F33Y-CuBMb variants in 100 mM phosphate buffer (pH6) containing 258 μM O2, 1 mM TMPD, and 10 mM ascorbate. (B) O2 reduction turnover measured during the stepwise addition of O2 for F33Y-CuBMb and its (MF-heme) and (DF-heme) variants.
